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Textile Research Journal
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A Dehydration Method for Immature or Wet Cotton Fibers for Light and Electron Microscopy

Kanniah Rajasekaran

USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, U.S.A., krajah{at}srrc.ars.usda.gov

Alan J. Muir

USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, U.S.A.

Bruce F. Ingber

USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, U.S.A.

Alfred D. French

USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, U.S.A.

A rapid dehydration procedure with acidified 2,2-dimethoxypropane (DMP) has been successfully applied to immature or wet cotton fibers. The ultrastructural integrity and fiber quality parameters such as cell wall area and perimeter of developing and mature cotton fibers were well-pre-served using this procedure as demonstrated by light, scanning and electron microscopy of whole fibers and cross-sections. Embedding in resin following DMP dehydration also prevented the collapse of the immature fibers due to drying of lumen.

Key Words: Cell wall • 2,2-dimethoxypropane • fiber quality • maturity • moisture • thin sections

Textile Research Journal, Vol. 76, No. 6, 514-518 (2006)
DOI: 10.1177/0040517506063390
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